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. 2020 Sep 9;10:1742. doi: 10.3389/fonc.2020.01742

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Copyright © 2020 Rozitis, Johnson, Cheng and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A–C) Subtypes of mesothelioma. (A) Epithelioid, (B) sarcomatoid, (C) biphasic. Epithelioid subtype with BAP-1 (D), MTAP (E) immunohistochemistry (loss of nuclear and cytoplasmic staining respectively); and FISH (F) of MTAP/CDKN2A/CEP9 tricolor probes (MTAP – Aqua, CDKN2A – Red, CEP9 – Green) showing loss of MTAP and CDKN2A (inset normal signals for comparison). Images (A–E) were taken by Olympus microscope with 20× objective, image (F) was taken by ZEISS Axio.M2 microscope with 63× objective.