FIGURE 5.

ERK signaling pathway mediated secretion of inflammatory cytokines evoked by rMgPa induced eCypA. (A) Western blot analysis of the cell lysates obtained from SV-HUC-1 cells transfected with siRNA and then stimulated with rMgPa, GAPDH was used as the control. (B) The mRNA levels of IL-1β, IL-6, TNF-α, and MMP-9 in the SV-HUC-1 cells preincubated with 30 μM PD98059 or BAY 11-7082 for 1 h before being stimulated with 20 μg/mL rMgPa were measured at 24 h by qRT-PCR. Supernatants were correspondingly collected after 24 h and measured by ELISA. *p < 0.05, N stands for the untreated group.