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. 2020 Sep 9;11:2065. doi: 10.3389/fimmu.2020.02065

Figure 2.

Figure 2

α-Defensins promote B. fragilis colonization of mucosal niches in the gut. (A) The experimental scheme for B. fragilis colonization in SPF mice. (B) B. fragilis colonization in Mmp7+/+ and Mmp7−/− littermates were analyzed by qPCR (n = 8–10). (C,D) Fluorescent immunohistochemistry analysis of B. fragilis in the small intestine (C) and colon (D) from Mmp7+/+ and Mmp7−/− littermates 2 days post gavage. Scale bar, 50 μm. The number of B. fragilis per 0.01 mm2 (0.1 × 0.1 mm; the white line indicates the linear distance of 0.1 mm) area from the surfaces of small intestine and colon (n = 4–5). Data are pooled from multiple independent experiments (B). Data are shown as mean ± SEM. Student t-test was performed; **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Multiple tissues sections were from each mice, and each group contains more than 3 individual mice on average in (C,D).