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. 2020 Sep 9;10:543562. doi: 10.3389/fonc.2020.543562

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Copyright © 2020 Zheng, Zhou, Qu, Ren, Yan, Guo and Yue.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Elevated VEGFR2 expression in osteosarcoma lung metastasis. (A) VEGFR2 and PD-L2 expression in 18 pairs of primary osteosarcoma specimens and pulmonary metastasis specimens was investigated via immunohistochemistry. Representative images are presented (magnification at 200×). (B) IHC scores of VEGFR2 and PD-L2 were analyzed between the primary osteosarcoma group and the pulmonary metastasis group. (C) The correlation between PD-L2 and VEGFR2 expression levels in 18 osteosarcoma pulmonary metastases was investigated. (D) VEGFR2 was overexpressed in osteosarcoma in comparison to normal control. Data are shown as the mean ± S.D. *p < 0.05, **P < 0.01.