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VEGFR2 inhibition attenuates osteosarcoma cell metastasis in vivo. (A,B) The quantities of pulmonary metastases in the apatinib and DMSO groups or shNC and shVEGFR2 groups are presented. Representative images are shown. (C,D) Local primary osteosarcoma was further estimated by immunoblotting and IHC (magnification at 400×) for PD-L2, p-STAT3 and p-LIMK expression. (E) A schematic drawing of VEGFR2 in osteosarcoma metastasis. (mean ± S.D., n = 4) **p < 0.01.
