Figure 1.

FASN Is Highly Expressed in Undifferentiated hPSCs

(A) Heatmap of proteomic analysis of undifferentiated hiPSCs (253G4) and hiPSC (253G4)-CMs (n = 3).

(B) Volcano plot of proteomic analysis of undifferentiated hiPSCs (253G4) and hiPSC (253G4)-CMs. Protein levels were transformed to log10 scale. Student's t test was performed for each protein (n = 3).

(C) Relative abundance of enzymes involved in glycolysis, TCA cycle, and de novo FA synthesis compared with hiPSC (253G4)-CMs in proteomic analysis. Student's t test was performed for each protein (n = 3).

(D and E) (D) and (E) Representative image of immunocytochemistry of cocultured hiPSCs (253G4) and hiPSC (253G4)-CMs. Scale bars, 100 μm.

(F) Representative image of western blot of hiPSCs (253G4) and hiPSC (253G4)-CMs.

Data are presented as means ± SD; ∗p < 0.05.