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. 2020 Jul 20;319(3):G323–G332. doi: 10.1152/ajpgi.00178.2020

Fig. 3.

Fig. 3.

Effect of transfection of PAC 266-6 with miR-200a-3p on thiamin uptake, mouse thiamin transporter-1 (mTHTR-1) protein and mRNA levels. Cells from the pancreatic acinar cell line 266-6 (PAC 266-6) were transfected with negative control mimic or mimic miR-200a-3p for 48 h. A: carrier-mediated thiamin uptake. B: expression of mTHTR-1 protein was determined (graph i); gel image showing the specificity of anti-THTR-1 polyclonal antibodies in PAC-266–6 (graph ii). C: expression of mTHTR-1 mRNA levels were determined. Data are means ± SE of 3–6 independent determinations (**P < 0.01; *P < 0.05).