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. 2020 Jul 20;319(3):G323–G332. doi: 10.1152/ajpgi.00178.2020

Fig. 4.

Fig. 4.

Effect of transduction of human primary pancreatic acinar cells (hPACs) with adenoviral miR-200a-3p on thiamin uptake, human thiamin transporter-1 (hTHTR-1) protein, and mRNA. A: bright-field (image i) and green fluorescent microscopic images (image ii) of hPACs transduced with adenovirus carrying green fluorescent protein (GFP). B: hPACs were transduced with miRNAs (Ad-control or Ad-200a-3p, 48 h), and carrier-mediated thiamin uptake was examined. C and D: hTHTR-1 protein in hPAC (graph i) and gel image showing specificity of anti-THTR-1 polyclonal antibodies (image ii) in Caco-2 cells (C) and mRNA (D) expression levels were determined. Data are means ± SE of 3–6 independent determinations (**P < 0.01; *P < 0.05).