Fig. 7.

Effect of mutating the miR-200a-3p putative binding site in the SLC19A2 3′-untranslated region (UTR) on luciferase activity in the pancreatic acinar cell line 266-6 (PAC 266-6). A: putative miR-200a-3p-binding site in SLC19A2 3′-UTR [wild-type (WT)] and mutated (MUT; substitution mutation is underlined). B: luciferase activity was measured in PAC 266-6 transfected with MUT construct; the activity was compared with cells transfected with WT after 48 h. C and D: luciferase activity was measured on WT or MUT SLC19A2 3′-UTR in PAC 266-6 cotransfected with mimic/inhibitor of miR-200a-3p compared with negative controls WT mimic/WT inhibitor. All luciferase activities were measured and normalized with Renilla luciferase activities. Data are means ± SE of 3–6 independent determinations (**P < 0.01; *P < 0.05). NS, not significant.