Table 1.

Sequence of the primers used in current investigation

Primer Name	Forward and Reverse Primer Sequences (5′-3′)
Primers used for cloning SLC19A2 3′-UTR in pmirGLO vector
SLC19A2 3′-UTR	ATTGAGCTCTATACTGCTGAAGGGCTTC; CAGCTCGAGTTTTGATTAAAAAGAGAAAATATAC
Real-time PCR primers
hTHTR-1	GCCAGACCGTCTCCTTGTA; TAGAGAGGGCCCACCACAC
hβ-actin	CATCCTGCGTCTGGACCT; TAATGTCACGCACGATTTCC
mTHTR-1	GTTCCTCACGCCCTACCTTC; GCATGAACCACGTCACAATC
mβ-actin	ATCCTCTTCCTCCCTGGA; TTCATGGATGCCACAGGA
Primers used for site-directed mutagenesis
	GGTATTAACTTTTTTAGAATGACACAGCTGAAAATTATCAATAATAGTGTGTCAAGATTGCAAAGTCGACA;
	TGTCGACTTTGCAATCTTGACACACTATTATTGATAATTTTCAGCTGTGTCATTCTAAAAAAGTTAATACC

Restriction sites are underlined, and mutated sites are in boldfaced italics; hTHTR-1, human thiamin transporter-1; hβ-actin, human β-actin; mβ-actin, mouse β-actin; mTHTR-1, mouse thiamin transporter-1; UTR, untranslated region.