American lifestyle-induced obesity syndrome (ALIOS) diet-fed mice have normal glucose tolerance. Intraperitoneal glucose tolerance tests (ipGTTs) were performed on normal chow (NC)- and ALIOS-fed male (A) and female (B) mice at 15, 25, 37, and 49 wk. ipGTTs are represented as areas under the curve (AUCs) in male (C) and female (D) NC- and ALIOS-fed mice at 15, 25, 37, and 49 wk (w). AUCs of serum insulin collected at t = 0, 60, and 120 min during the ipGTT in male (E) and female (F) mice are shown. Data are expressed as means ± SE. n = 7 mice in each group at each time point; *P < 0.05, **P < 0.01.