American lifestyle-induced obesity syndrome (ALIOS) diet drives hepatic steatosis. Liver mass of male (A) and female (B) and hepatic triacylglycerol (TAG) content in male (C) and female (D) normal chow (NC)- and ALIOS-fed mice at 26 and 52 wk (w; 26 wk: NC males, n = 12; ALIOS males, n = 15; NC females, n = 15; ALIOS females, n = 15; 52 wk: NC males, n = 17; ALIOS males, n = 12; NC females, n = 15; ALIOS females, n = 15) are shown. Representative hematoxylin-eosin images are from male mice at 52 wk fed NC (E) and ALIOS (F) depicting macro- and microvesicular lipid droplets. Data are expressed as means ± SE. Scale bar is 25 µm. ALIOS-fed male and female mice had altered expression of genes associated with lipid metabolism (G, males; H, females) and insulin signaling (I, males; J, females). n = 8 Mice in each group. Genes are expressed as fold changes in log10 compared with NC. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. BW, body weight.