Table 1.

Description of MLP-1 constructs

Description	Name	Mutations	Phenotype
Wild-type protein	WT	None	Normal function
Three protein kinase C phosphorylation target serines in the effector domain changed to alanines	S3A	S93A, S101A, S104A	Cannot be phosphorylated by PKC
Three protein kinase C phosphorylation target serines in the effector domain changed to aspartic acid	S3D	S93D, S101D, S104D	Acts like constitutively phosphorylated
Myristoylation site changed to alanine	GA	G2A	Cannot be myristoylated

MLP-1, myristoylated alanine-rich protein kinase C substrate-like protein-1.