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. 2020 Jul 27;319(3):F476–F486. doi: 10.1152/ajprenal.00594.2019

Fig. 1.

Fig. 1.

Systemic inhibition of miR-451 reduces renal miR-451 expression. A: kidney whole homogenate expression of miR-451 (data normalized to RNU6) in scrambled locked nucleic acid (LNA; scramble)-treated TallyHo/Jng (TH), LNA-miR-451 inhibitor-treated TH, and untreated C57Bl6 male mice. In situ hybridization using a nonspecific probe (B) and using a specific miR-451 probe (C–H) is shown. C and D: cortex (C) and medulla (D) from an LNA scramble-treated TH mouse. E and F: cortex (E) and medulla (F) from an LNA-miR-451 inhibitor-treated TH mouse. G and H: cortex (G) and medulla (H) from an untreated C57Bl6 mouse (×400). Yellow arrows point to erythrocytes that have high levels of miR-451 in scramble-treated mice. CD, collecting duct; PT, proximal tubule; VR, vasa recta; GLM, glomerulus; DT, distal tubule; DCT, distal convoluted tubule. *Tukey’s multiple-comparison test was used to determine significance, with P < 0.05 considered significant (n = 8 mice/group).