Fig. 4.

Systemic inhibition of miR-451 increases predicted targets of miR-451 in the cortex of TallyHo/Jng (TH) mice. A and B: Western blot analysis (A) and relative density summaries (B) for tyrosine 3-monooxygenase/tryptophan 5-monooygenase activation protein-ζ (YWHAZ) and matrix metallopeptidase-9 (MMP-9) (normalized to Ponceau red stain). C: representative YWHAZ staining showing the increase in YWHAZ protein in glomeruli [mesangium (white arrows)] of inhibitor-treated mice. Scale bar = 50 µm. LNA, locked nucleic acid. Data are presented as means ± SE. *An unpaired t test was used to determine significance, with P < 0.05 considered significant.