Fig. 5.

Systemic inhibition of miR-451 increases fibrotic and mechanistic target of rapamycin (mTOR) signaling in the cortex. A and B: Western blot analysis (A) and relative (normalized to Ponceau stain) density summaries (B) for mTOR, collagen type IV (Col IV), AMP-activated protein kinase-α (AMPKα), transforming growth factor-β (TGF-β), and calcium-binding protein 39 (CAB39). C: representative mTOR immunohistochemistry (IHC) in scramble- and inhibitor-treated mice. LNA, locked nucleic acid. Data are presented as means ± SE; n = 8 mice/group. An unpaired t test was used to determine significance, with P < 0.05 considered significant. *P < 0.05.