Fig. 8.

Validation of direct targets of miR-451 in human proximal tubule (hPT) cells. hPT cells were brought to confluence and then treated with locked nucleic acid (LNA) miR-451a inhibitor or negative control at 50 nM. After 48 h, transfected cells were harvested, total RNA was prepared, and quantitative RT-PCR was conducted. A: fold expression ($2^{-\Delta {\text{C}}_{\text{T}}}$; where C_T is threshold cycle) of miR-451, tyrosine 3-monooxygenase/tryptophan 5-monooygenase activation protein-ζ (YWHAZ), and calcium-binding protein 39 (CAB39) in hPT cells transfected with either Homo sapiens (hsa-)LNA-miR-451a-inhibitor or hsa-LNA-miR-451a inhibitor control (LNA scramble). Transcript levels of genes of interest were normalized against 18S rRNA. *Significant difference due to an unpaired t test (P < 0.05). B: representative immunofluorescence image of hPT cells stained for aquaporin-1 protein (green) and counterstained with DAPI (blue, nuclear stain). White lines represent scale bars of 50 μm. Images were taken at ×100 magnification.