Table 1.

Predicted versus detected NPM phosphorylation sites

	Site Number
Phospho Sites	Potential	Predicted	Detected
Serines	26	19	6
Threonines	10	5	3
Tyrosines	4	2	0
Total	40	26	9

The total number of serine, threonine, and tyrosine residues in human and murine nucleophosmin (NPM) is identical. Of the 40 total NPM residues available for phosphorylation, computational analysis predicts that fewer residues will be phosphorylated based on >50% likelihood. In fact, mass spectrometry revealed that only 9 of 26 predicted residues were actually phosphorylated either at baseline or after ischemia in the human kidney.