FIGURE 2.
Effect of RPL21 knockdown on DNA replication and cell cycle progression of PANC-1 and BxPC-3 cells. (A,B) The PANC-1 and BxPC-3 cells after transfection of siRNAs (Mock-siRNA, siL21-1 and siL21-2) (40 nM, 72 h) were incubated with 50 μM of 5-ethynyl-2′-deoxyuridine (EdU). Then the cells were stained with Hoechst 33342 for 30 min and examined by laser scanning confocal microscopy (magnification: ×400). (C,D) Summary graphs of the EdU retention assay for PANC-1 and BxPC-3 cells. (E,G) Human pancreatic cancer cells (PANC-1 and BxPC-3) were seeded in 6-well plates (2 × 105 cells/well) and treated with Mock-siRNA, siL21-1 and siL21-2 (40 nM) for 72 h. Cells were harvested at the indicated time point post transfection and stained with propidium iodide (PI) for DNA cell cycle analysis. (F,H) Percentage of cell cycle distribution. Three independent experiments were of similar results. The untransfected cells serves as a control, and the Mock-siRNA transfected cells serves as a negative control (NC). Each bar represents the mean ± SD of triplicate analysis. * indicates P < 0.05 compared to the control as determined by the Student’s t-test.