FIGURE 5.

Prickle2 treatment reduces Tau phosphorylation in 3 × Tg mice. (A) Representative IHC images of WT, 3 × Tg and 3 × Tg/Prickle2 treatment mice stained with anti-Tau (phospho S202) antibodies. The proportions of pS202-positive cells were calculated in cortical and hippocampal, respectively (n = 6 per group). (B) Representative IHC images of WT, 3 × Tg and 3 × Tg/Prickle2 treatment mice stained with anti-Tau (phospho S396) antibodies. The proportions of pS396-positive cells were calculated in cortical and hippocampal, respectively (n = 6 per group). (C) Western blots detected the phosphorylated Tau using pS202 and pS396 antibodies in the brain tissues from WT, 3 × Tg and 3 × Tg/Prickle2 mice. (D–F) Quantification of the protein levels of phosphorylated Tau by densitometric analyses (n = 6 per group). (B–D) ANOVA followed by Bonferroni’s post hoc test. ^∗∗p < 0.01; ^∗∗∗p < 0.001; ^*⁣*⁣**p < 0.0001.