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The MFG number throughout the estrous cycle. Milk samples were stained with Nile red and the milk fat globule (MFG) number was recorded. Five microscopic fields were counted for each sample (i.e., from each cow; n = 12). The MFG number varied between 101 and 196 droplets per sample along the estrous cycle. Higher number of MFG was observed on days 1 and 2 post-ovulation (p ≤ 0.0001).
