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. 2020 Sep 9;11:531785. doi: 10.3389/fimmu.2020.531785

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Copyright © 2020 Zhou, Xing, Tang, Sheng, Chi and Zhan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Blockade assay analysis of CD4-1+ T lymphocytes, CD4-2+ T lymphocytes, and IL-2Rβ+ cells in the peripheral blood of flounder by FCM. (A) The representative dots of show the results for CD4-1+ T lymphocytes, CD4-2+ T lymphocytes, and IL-2Rβ+ cells in anti-frIL-2 and anti-frIL-2β polyclonal antibody groups at 72h post addition. FCM was used to analyze the stained cells. (B) Percentages of CD4-1+ T lymphocytes, CD4-2+ T lymphocytes, and IL-2Rβ+ cells in different groups. All values are expressed as mean ± SD from the six of independent experiments.