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. 2020 Sep 9;11:531785. doi: 10.3389/fimmu.2020.531785

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Copyright © 2020 Zhou, Xing, Tang, Sheng, Chi and Zhan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SDS-PAGE confirmed binding between His-rfIL-2 and GST-rfIL-2Rβ by pull-down assay. Lane M, Molecular mass marker; Lane 1, protein profile of BeaverBeads^TM GSH beads coupled with purified GST-rIL-2Rβ and His-rIL-2. Lane 2, protein profile of BeaverBeads^TM GSH beads coupled with purified GST-rIL-2Rβ and PBS. Lane 3, BeaverBeads^TM GSH beads coupled with purified GST-labeled protein and His-rfIL-2. Lane 4, BeaverBeads^TM GSH beads coupled with PBS and His-rfIL-2 as negative control.