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. 2020 Sep 9;11:531785. doi: 10.3389/fimmu.2020.531785

FIGURE 8.

FIGURE 8

FCM assay investigating the proportion of CD4-1+ T lymphocytes, CD4-2+ T lymphocytes, and IL-2Rβ+ cells in peripheral blood of flounder cultured in vitro after rIL-2 addition at 72 h. (A) The representative dots of the results for CD4-1+ T lymphocytes, CD4-2+ T lymphocytes, and IL-2Rβ+ ells from PBS, PHA + rHis, and PHA + rHis-IL-2 groups at 72 h post addition. FCM was used to analyze the stained cells. (B) Percentages of CD4-1+ T lymphocytes, CD4-2+ T lymphocytes, and IL-2Rβ+ cells in different groups. All values are expressed as mean ± SD from the six of independent experiments. Asterisks (*) above the bar represent the statistical significant difference, *p < 0.05, **p < 0.01.