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. 2020 Sep 9;11:531785. doi: 10.3389/fimmu.2020.531785

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Copyright © 2020 Zhou, Xing, Tang, Sheng, Chi and Zhan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative gene expression in PBLs of flounder after addition of PBS, PHA + rHis, and PHA + rHis-IL-2 at 72 h. (A) The gene expression profile of T cell markers (CD3, CD4-1, CD4-2, and CD8β). (B) The gene expression profile of related cytokines (IL-2, IL-2Rβ, TNFα, IFNγ, IL-12p40, and IL-10), transcription factors (T-bet and GATA3). (C) The gene expression profile of IL-2 pathway (AKT, JAK1, PI3K, SHC1). The results represent the mean ± SD from the three independent experiments. Different letters above the bar represent the statistical significance (p < 0.05).