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. 2020 Sep 15;20(5):245. doi: 10.3892/ol.2020.12107

Figure 2.

Figure 2.

miR-519 directly binds to HIF-1α. (A) Bioinformatics analysis results demonstrating the binding site between miR-519 and HIF-1α. (B) Dual luciferase reporter assay of the luciferase activity of wild-type and mutant 3′-untranslated region of HIF-1α in 293T cells transfected with NC, miR-519 mimics and miR-519 inhibitor. (C) Reverse transcription-quantitative PCR results of miR-519 and HIF-1α expression levels in SUNE1 and SUNE2 cells transfected with NC, miR-519 mimics and miR-519 inhibitor. (D) Western blotting results of the expression of HIF-1α in SUNE1 and SUNE2 cells transfected with NC, miR-519 mimics and miR-519 inhibitor. GAPDH was the loading control. Data are presented as the mean ± SD. *P<0.05 and **P<0.01. miR, microRNA; NC, negative control; HIF-1α, hypoxia-inducible factor-1α.