Figure 3.

LncAFAP1‐AS1 induces radioresistance of TNBC via activating the Wnt/β‐catenin signaling pathway. A) The silver staining and B) Western blot analysis of the RNA‐protein binding complexes after RNA pulldown of lncAFAP1‐AS1 in MDA‐MB‐231R cells. C) RIP of β‐catenin in MDA‐MB‐231R cells. LncAFAP1‐AS1 was retrieved by β‐catenin or IgG and then detected by qRT‐PCR. D) Western blot analysis of the expression of CK1, GSK3β, p‐GSK3β, and active and total β‐catenin in MDA‐MBA‐231R cells treated with the Lipo2k/si‐1, Lipo2k/si‐2, or Lipo2k/siCTL complexes at a siRNA concentration of 30 × 10⁻⁹ m. E) Schematic illustration of the molecular mechanism of lncAFAP1‐AS1 to induce TNBC radioresistance via suppressing the function of β‐catenin destruction complex and subsequently activating the Wnt/β‐catenin signaling pathway to promote TNBC cell proliferation, migration, and invasion.