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. 2020 Jul 21;7(18):2000843. doi: 10.1002/advs.202000843

Figure 6.

Figure 6

Transfer of cargo‐loaded spiral between different environments: A) Capture of murine zygote inside of trimmed 10 µL pipette tip; B) transfer of cargo‐loaded spiral to PDMS channel by pipetting; C) transport of zygote to other end of channel by magnetic actuation (i–iii); D) transfer of cargo‐loaded spiral to Petri dish by pipetting and release of zygote by magnetic actuation; E) fluorescence staining indicating viability of one cleaved zygote after capture (time taken: 4 min), magnetic manipulation (time taken: 8 min), and 24 h of incubation while inside the spiral in PDMS channel; F) successful cell division of one zygote, depicting the zygote after capture (time taken: 3 min) and magnetic manipulation for 3 min (i), and after 24 h of incubation while inside the spiral (ii) in PDMS channel (yellow arrows mark a piece of debris of spiral's metal coating that indicate that indeed the same spiral is depicted); all scale bars are set at 100 µm except in (B) 1 mm, and in (D) Petri dish diameter is 3 cm—spiral and zygote are displayed in magnified insets.