Table 2.

Parameters estimation for each variable in the CRC cohort

	Threshold		Specificity		Sensitivity		AUC
Parameter	Estimation	IC95%	Estimation	IC95%	Estimation	IC95%	Estimation	IC95%
CRC versus healthy
Ref A 67	3484	[3076–4857]	0.82	[0.77–0.87]	0.74	[0.69–0.78]	0.83	[0.81–0.86]
Ref A 145	1491	[1121–1639]	0.79	[0.70–0.86]	0.74	[0.69–0.82]	0.83	[0.80–0.86]
Ref A 320	425	[286–509]	0.77	[0.69–0.83]	0.67	[0.62–0.74]	0.76	[0.73–0.79]
MNR	12 103	[6945–15 360]	0.73	[0.65–0.82]	0.70	[0.61–0.76]	0.78	[0.75–0.82]
Ref M 67	4.41E+07	[2.78E+07–6.04E+07]	0.60	[0.46–0.69]	0.57	[0.49–0.69]	0.59	[0.56–0.63]
Patients paired by age category CRC versus healthy
Ref A 67	3726	[2921–4511]	0.806	[0.72–0.89]	0.7	[0.62–0.78]	0.786	[0.73–0.84]
Ref A 145	16 113	[1267–1874]	0.793	[0.67–0.91]	0.715	[0.62–0.84]	0.794	[0.74–0.87]
Ref A 320	617	[100–899]	0.763	[0.67–0.92]	0.631	[0.53–0.73]	0.631	[0.53–0.73]
MNR	12 189	[4295–16 225]	0.713	[0.61–0.81]	0.707	[0.59–0.82]	0.748	[0.69–0.81]
Ref M 67	4.78E+07	[3.54E+07–6.87E+07]	0.602	[0.45–0.69]	0.618	[0.52–0.78]	0.606	[0.53–0.67]
Patients paired by age category early stages (0‐I‐II) versus healthy
Ref A 67	3415	[1800–4321]	0.747	[0.57–0.91]	0.64	[0.49–0.80]	0.694	[0.58–0.82]
Ref A 145	1542	[1012–1874]	0.753	[0.57–0.92]	0.672	[0.53–0.84]	0.725	[0.62–0.85]
Ref A 320	502	[72–706]	0.719	[0.54–0.90]	0.651	[0.49–0.82]	0.689	[0.58–0.81]
MNR	15 022	[6960–21 942]	0.676	[0.51–0.84]	0.664	[0.51–0.84]	0.668	[0.55–0.78]
Ref M 67	4.71E+07	[1.16E+07–7.13E+07]	0.639	[0.43–0.81]	0.576	[0.40–0.74]	0.571	[0.45–0.68]

a, Parameters estimation for each variable based on minimum distance between corresponding ROC curve and the point (Se = 1, 1‐Sp = 0) with the use of an empirical bootstrap to build their respective confidence interval. These estimations were obtained before and after age adjustment with a focus on early stages (0‐I‐II) colorectal cancer patients. b, Se = sensitivity; c, Sp = specificity; d, AUC = area under curve.