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. 2020 Jul 29;7(18):2000515. doi: 10.1002/advs.202000515

Figure 4.

Figure 4

Characterization of the antitumor effect of gETL NPs. a) Characterization of drug release behavior of gETL NPs at the indicated temperatures. b) Schematic illustration of the experiment to characterize the antitumor effect of gETL NPs in combination with Pt thermos‐chemotherapy under the simulated working conditions of HIPEC in vitro. c) Viability of CT26 cells treated with gETL NPs, DTX‐liposomes, Pt at 37 °C. d) Viability of CT26 cells treated with gETL NPs, DTX‐liposomes, Pt at 42 °C. e) Flow cytometry analysis of the apoptosis of CT26 cells treated with gETL NPs, DTX‐liposomes, Pt at 42 °C was detection by flow cytometry. f) Quantitative analysis of apoptosis based on flow cytometry. The data was presented as the mean ± SD from three repeated experiments, One‐way ANOVA was used to determine statistical differences. (*p < 0.05, **p < 0.01, ns: not significant). Lips, DTX‐liposomes; NPs, GM‐CSF and DTX loaded gETL NPs; Pt, Oxaliplatin.