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. 2020 Jul 29;7(18):2000515. doi: 10.1002/advs.202000515

Figure 9.

Figure 9

Characterization of the antitumor mechanism. a) Fold‐changes of TNF‐α concentration in tumor homogenate and serum compared with control group. b) Fold‐changes of IFN‐γ concentration in tumor tissue homogenate and serum compared with control group. c) Quantitative analysis of the ratio of M1 macrophages (CD86+)/M2 macrophages (CD206+) by immunofluorescence staining of tumor sections. d) Quantitative analysis of tumor infiltrating T cells (CD3+), CD4 T cells (CD3+ CD4+), and CD8 T cells (CD3+CD8+) of mPC tumors. Mean count of positive cells from 5 high‐power field per section was used for statistical difference analysis, One‐way ANOVA was used to determine statistical differences; ♦, p < 0.05, ♦♦, p < 0.01, ♦♦♦, p < 0.001, compared with control group; *p < 0.05; **p < 0.01, ***p < 0.001, compared with the indicated groups. e) Immunofluorescence staining of different macrophages markers (CD68+ CD86+ or CD68+ CD206+) and T cells markers (CD3+, CD4+, CD8+) in tumor tissue sections. Scale bar: 100 µm.