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. 2018 Sep 27;1(1):26–41. doi: 10.3390/clockssleep1010004

Figure 3.

Figure 3

Comparing phase-shifting responses to different 120 s light fractionation regimens. Representative actograms taken from flies receiving no light (Row 1) or either: continuous light for 15 min (Protocol A, Row 2), 15 s of light every 2 min (Protocol F, Row 3), a series of four 30 s pulses spaced 90 s apart (Protocol H, Row 4), a series of eight 15 s pulses spaced 15 s apart (Protocol I, Row 5), or stimulation with two pairs of 30 s pulses (90 s ISI) bookending the 15-min delivery window (Protocol K, Row 6). All protocols began precisely at ZT23 and were completed by ZT23.25. Post treatment, animals were left to free-run in constant darkness (DD) for 4 days. Grey and black bars show the timing of the previous LD schedule, while asterisks and a dotted line mark the timing of light administration. Example actograms from each protocol are organized in temporally aligned columns to help visualize shifts in locomotor rhythms.