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. 2018 Sep 27;1(1):26–41. doi: 10.3390/clockssleep1010004

Figure 4.

Figure 4

Illustration of light fractionation protocols with approximately 75 s total exposure. After lights-off on the last day of a 12:12 LD schedule at ZT23-ZT23.25 (grey box), separate groups of flies received either a 15-min pulse of uninterrupted, constant light (A), or intermittent delivery of light according to the following logic: (L) stimulation for 15 s every 3 min, (M) stimulation with five 15 s pulses positioned 15 s apart, (N) stimulation with four 19 s pulses positioned 57 s apart, (O) stimulation with three 25 s pulses positioned 75 s apart, (P) stimulation for 25 s every 5 min, (Q) stimulation for 25 s every 6 min, (R) stimulation for 25 s every 7 min, or (S) stimulation for 76 s within two symmetrical 38 s blocks distributed at the tail-ends of ZT23-ZT23.25.

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