Figure 10.

BP nanosheets with autophagy‐inhibiting capabilities for cancer therapeutic applications. a) Confocal fluorescence images of HeLa and MCF‐7 cells expressing mRFP‐GFP‐LC3 after treatment with BP nanosheets for 24 and 48 h. Aberrant autophagosome accumulation (yellow) can be visualized, which attribute to the lysosomal blockage by BP nanosheets. Scale bar, 10 µm. b) Immunoblot analyses for the expressions of p62 and LC3 in HeLa and MCF‐7 cells after treatments with BP nanosheets for 24 and 48 h. β‐actin was used as a loading control. Reproduced with permission.^{[ 36 ]} Copyright 2019, Wiley‐VCH. c) Schematic illustration for the cellular mechanism of the synergy between 2DG and BP nanosheets that enables concurrent glycolysis inhibition and autophagy blockage for exacerbating cancer cell starvation. Reproduced with permission.^{[ 39 ]} Copyright 2020, Wiley‐VCH.