Figure 5.

Human observer validation. (a) Sum of licking in seconds was calculated for 2 min for each of 111 videos for both Observer 1 (red square) and the model (blue circle). The videos are displayed ranked from low to high levels of licking ordered by the human observations. The boxes I–IV represent an example of categorization of behavior response level imposed on the data with approximately a 20-s separation: (I) very low level (human mean 1.6 s: model mean 2.5 s), (II) low-mid level (human mean 20.7: model mean 22.9), (III) moderate level (human mean 35.6: model mean 38.2), and (IV) high level (human mean 63.3: model mean 62.2). Observer 2 annotated 14 of these videos and the summed results for each (green open square) are superimposed on the plot of Observer 1 rankings (located at rank: 65, 73, 82, 83, 87, 88, 94, 95, 100, 102, 106, 107, 108, 110). Mice ranked at 95 and 110 have identical scores, and the markers are therefore indistinguishable, scores for mice at 73, 82, and 106 differ by only 1 s for one of the marks. (b) to (d) Comparison of manual scoring by two human operators with the classifier model for three mice. The data are shown as summed licking in 5 min bins for 60 min postinjection (bins range from 5–10 to 55–60).