Figure 6.

Intranasal immunization with CS‐IONzyme‐based influenza vaccine protects mice against H1N1 influenza virus infection. The vaccinated mice were challenged with 10⁶ EID₅₀ of H1N1 influenza virus 28 days post primary immunization. a) Body weight changes. b) Survival rates. Mice who lost more than 25% of their body weight were euthanized according to guidelines. n = 10 mice per group. c) Virus load in the lung (n = 5 per group) on day 5 p. i. d) The pathological changes in lungs (n = 3 per group) on day 7 p. i. e) Representative histopathological changes in H&E‐stained lung tissues (n = 3) on day 7 p. i. Bars: 500 µm. f) Histopathologic scores in the lungs. Data shown represent the means ± s.d. Results are from one representative experiment of two performed. Statistical significance is assessed by One‐way ANOVA analysis to compare the results between different groups. *P < 0.05; **P < 0.01.