Table 2.
Antibodies Used in this Study
| Antigen | Species | Specific for | Dilution | Supplier | Catalogue # | RRID |
|---|---|---|---|---|---|---|
| Primary antibodies | ||||||
| Brn3b | Rabbit | Ganglion cells (transcription factor POU4F2) | 1:100 (fl.Ab) | Abcam (Eugene, OR) | ab56026 | AB_880587 |
| Chx10/Vsx2 | Sheep | Retinal progenitors (transcription factor) | 1:200 (fl.Ab) | Thermo Fisher (Huntington Beach, CA) | PA1-12565 | AB_2216011 |
| CRALBP | Rabbit | RPE and Muller cells | 1:1K (fl. Ab) | Dr. John Saari (Univ. of WA)60 | N/A | AB_2314227 |
| CRALBP | Mouse | RPE and Muller cells | 1:500 (fl. Ab) | Abcam | ab15051 | AB_2269474 |
| RLBP1(=CRALBP) | Rabbit | RPE and Muller cells | 1:200 (fl. ab) | Fitzgerald (North Acton, MA) | 70R-19908 | N/A |
| CRX | Rabbit | Photoreceptor progenitor | 1:200 (fl.Ab) | Santa Cruz Biotechnology, | sc-30150 | AB_2276566 |
| GFAP (Clone GA-5) | Mouse | Reactive glial cells | 1:1K (fl. ab) | Sigma | G3893 | AB_477010 |
| GFAP (Clone GA-5) | Mouse | Reactive glial cells | 1:1K (fl. ab) | Calbiochem/Millipore | IF03L | AB_212974 |
| GFAP (Clone GA-5) | Mouse | Reactive glial cells | 1:100 (fl. Ab) | Cell Signaling Technology (Danvers, MA) | #3670 | AB_561049 |
| Glutamine synthetase | Mouse | Müller cells | 1:2K (fl. Ab) | Transduction labs/BD Biosciences, San Jose CA | 610518 | AB_397880 |
| Iba1 | Rabbit | Microglia | 1:100 (fl. Ab)1:500 (ABC) | Biocare Medical (Pacheco, CA) | 901-290-031218 | N/A |
| Ku80 | Rabbit | Human nuclei | 1:400 (fl. Ab)1:2K (ABC) | Abcam | ab80592 | AB_1603758 |
| MAP2 | Mouse | Ganglion cells | 1:200 (fl.Ab) | Millipore | MAB3418 | AB_94856 |
| Otx2 | Rabbit | Homeobox transcription factor in retinal and forebrain development | 1:500 (fl.Ab) | Chemicon/Millipore | AB9566 | AB_2157186 |
| PKC α | Mouse | Rod bipolar cells | 1:50 (fl. Ab) | Amersham (Little Chalfont, U.K.) | N/A (purchased 1990) | N/A |
| Recoverin | Rabbit | Photoreceptors, cone bipolar cells | 1:2K (fl. Ab)1:10K (ABC) | Millipore | AB5585-I | AB_2253622 |
| Red/green opsin | Rabbit | Red and Green cones | 1:2K (fl. Ab) | Chemicon/Millipore | AB5405 | AB_177456 |
| Rhodopsin (rho1D4) | Mouse | Rods | 1:100 (fl. Ab)1:10K (ABC) | Dr. Robert Molday, Univ. of British Columbia61 | N/A | N/A |
| α-Synuclein; clone D37A6 | Rabbit | Rodent amacrine cells and IPL; OLM (general) | 1:100 (fl. Ab) | Cell Signaling Technology | 4179 | AB_1904156 |
| Synaptophysin | Goat | Membrane protein of synaptic vesicles | 1:100 (fl. Ab) | Novus Biologicals (Littleton, CO) | AF5555 | N/A |
| SC-121 (STEM121) | Mouse | Cytoplasm of human cells | 1:2K (fl. Ab)1:25K (ABC) | Stem Cell Inc. (Newark, CA) | AB-121-U-050 | AB_2632385 |
| Secondary antibodies | ||||||
| Alexa Fluor 488 | Donkey | Rabbit IgG (H+L) | 1:400 | Jackson Immuno Research (West Grove, PA) | 711-545-152 | AB_2313584 |
| Rhodamine Red-X | Donkey | Rabbit IgG (H+L) | 1:400 | Jackson Immuno Research | 711-295-152 | AB_2340613 |
| Alexa Fluor 647 | Donkey | Rabbit IgG (H+L) | 1:400 | Jackson Immuno Research | 711-605-152 | AB_2492288 |
| Biotin-SP | Donkey | Rabbit IgG (H+L) | 1:200 | Jackson Immuno Research | 711-065-152 | AB_2340593 |
| Alexa Fluor 488 | Donkey | Mouse IgG (H+L) | 1:400 | Jackson Immuno Research | 715-545-150 | AB_2340846 |
| Rhodamine Red-X | Donkey | Mouse IgG (H+L) | 1:400 | Jackson Immuno Research | 715-295-151 | AB_2340832 |
| Biotin-SP | Donkey | Mouse IgG (H+L) | 1:200 | Jackson Immuno Research | 715-065-140 | AB_2340783 |
| Alexa Fluor 488 | Donkey | Sheep IgG (H+L) | 1:400 | Jackson Immuno Research | 713-545-147 | AB_2340745 |
| Rhodamine Red-X | Donkey | Sheep IgG (H+L) | 1:400 | Jackson Immuno Research | 713-295-147 | AB_2340737 |
| Alexa Fluor 647 | Donkey | Sheep IgG (H+L) | 1:400 | Jackson Immuno Research | 713-605-003 | AB_2340750 |
| Biotin-SP | Donkey | Sheep IgG (H+L) | 1:200 | Jackson Immuno Research | 713-065-003 | AB_2340715 |
| Biotin-SP | Donkey | Goat IgG (H+L) | 1:200 | Jackson Immuno Research | 705-065-003 | AB_2340396 |
| Alexa Fluor 488 | Donkey | Goat IgG (H+L) | 1:400 | Jackson Immuno Research | 705-545-003 | AB_2340428 |
| Rhodamine Red-X | Donkey | Goat IgG (H+L) | 1:400 | Jackson Immuno Research | 705-295-003 | AB_2340422 |
| Alexa Fluor 647 | Donkey | Goat IgG (H+L) | 1:400 | Jackson Immuno Research | 705-605-147 | AB_2340437 |
CRALBP = cellular retinaldehyde binding protein, specific for Müller cells and RPE; GFAP = glial fibrillary acidic protein.