Figure 5.

Effects of pharmacologically altering zonular tension on the subcellular localization of AQP5 in the efflux zone of the rat lens. (A) Image montage of the water efflux zone taken from a representative axial section of a rat lens labeled with the membrane marker WGA (red) and the nuclei marker DAPI (blue). The box indicates the area from which high-resolution images (B–D) were captured in lenses maintained in situ with their zonules intact. (B–D) Top panels show nuclei, membrane, and AQP5 labeling, while bottom panels show only nuclei and AQP5 labeling from control lenses (B) and lenses incubated in tropicamide (C) or pilocarpine (D) for 60 minutes. Note the shift from membranous labeling to cytoplasmic labeling following incubation on pilocarpine.