Figure 8.

Subcellular distribution of AQP5 in lenses with pharmacologically modulated zonular tension at the anterior suture. (A) Image montage of the anterior water influx zone taken from an axial section of a rat lens that was labeled with the membrane marker WGA (red) to highlight suture line (arrowheads). Boxes indicate the areas (IA1, IA2, and IA3) from which the higher-resolution images shown in B and C were taken to investigate the subcellular distribution of AQP5 (green) following the application of tropicamide (B) or pilocarpine for 60 minutes. (B) Application of tropicamide did not change AQP5 labeling in the suture (compare to Fig. 6E). (C) In lenses treated with pilocarpine, AQP5 labeling was absent from the sutures of regions IA1 and IA2 but was still present at IA3.