Figure 2.

Expression of VEGF-B and its receptors VEGFR1 and NRP1 under different stress conditions. QMMuC-1 cells cultured under hypoxia (HYP, 1% O₂), high glucose (HG, 30 mM), or mannitol (MANN), IL-1β (20 ng/mL) and 4-HNE (10 µM) for 72 hours (for HYP and HG/MANN) or 24 hours (for IL-1β and 4-HNE) and the mRNA expression of VEGF-B (A), coreceptor NRP1 (D), and VEGFR1 (F) was determined by qPCR. Protein expression of VEGF-B in QMMuC-1 cell lysates (B) and supernatant (C), NRP1 and VEGFR1 (E and G, respectively) and the soluble receptor sFLT-1 was assessed by ELISA. n = 3–5 per group in PCR, n = 5-8 in ELISA. ^*P < 0.05; ^**P < 0.01; ^***P < 0.005 Student t test, compared with respective control.