Table 1.

Concentration of Compounds Used for Functionality Assays and to Induce Different Stress Conditions

Compound	Reference	Concentration/Length	Source	Catalog No.
VEGF-A murine recombinant	34 , 38	100 ng/mL	Peprotech	450-32
VEGF-B murine recombinant	33 , 34 , 37	100 ng/mL	Cloud-Clone Corp.	rpa144muo1
VEGF-A antibody	32	500 ng/mL	R&D. Polyclonal Goat IgG	af493
VEGF-B antibody	34 , 50	500 ng/mL	R&D. Polyclonal Goat IgG	af590
VEGFR1 antibody	33 , 35	10 µg/mL	R&D. Polyclonal Goat IgG	af471
VEGFR2 antibody	33 , 35	10 µg/mL	R&D. Polyclonal Goat IgG	af644
NRP1 antibody	54	30 µg/mL	R&D. Polyclonal Goat IgG	af566
Recombinant mouse IL-1β	29 , 30	20 ng/mL- 24 hours	R&D	401-ML
D-(+)-Glucose/ D-Mannitol	25 , 26	25mM- 72 hours*	Sigma Aldrich
Hypoxia	27 , 28	72 hours
4-HNE		10 µM† or 20 µM‡-24 hours	BioVision	2083-1
HC-067047	43 , 58 , 60	10 µM	Tocris Bioscience	4100
GSK1016790A		100 nM	Sigma Aldrich	G0798

^*No changes in cell viability at this length (data not shown).

^†Sublethal dose of 4-HNE chosen for study the effect of oxidative stress.

^‡Concentration of 4-HNE used in survival studies that induces approximately 15%–25% decrease in cell survival.