Table 3.
Antibodies Used for Western Blotting and Immunofluorescence
| Antibody | Company | Catalog Number | Dilution |
|---|---|---|---|
| AQP4 | Santa Cruz | SC-20812 | 1:500 |
| β-actin | Abcam | ab8224 | 1:6000 |
| GFAP | DAKO | z0334 | 1:800 |
| Kir4.1 | Abcam | AB105102 | 1:500 |
| GS | Sigma | G2781 | 1:2500 |
| Vimentin | Biorbyt | orb167196 | 1:1000 |
| TRPV4 | ProSci | 7695 | 1:500(WB)/1:50 (IF) |
| Caspase-3 | ProSci | PSI-3445 | 1:100(IF)/1:30 (IF) |
| AlexaFluor 488 donkey anti-rabbit | Abcam | ab150073 | 1:300 |
| Alexa Fluor 594 Phalloidin | ThermoFisher | A12381 | 1:10,000 |
| Goat Anti-Rabbit IgG H&L (HRP) | Abcam | ab6721 | 1:10,000 |
| Rabbit Anti-Mouse IgG H&L (HRP) | Abcam | ab6728 | 1:10,000 |
| Rabbit Anti-Goat IgG H&L (HRP) | Abcam | ab6741 | 1:7000 |