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. 2020 Sep 23;10:113. doi: 10.1186/s13578-020-00472-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — NRP2 upregulates cofilin activity by increasing SSH1 expression. A Heatmap of an NCBI dataset (GSE73514) of MLP tumors and ITs. Cluster analysis was performed according to an NRP2 transcription level difference of more than fivefold in 2 groups (red indicates high transcription levels, and blue indicates low transcription levels). a Cluster analysis results. b GSEA mountain plot showing a strong association between the MLP and IT groups. c, Correlation analysis of NRP2 and SSH1 transcription levels. B SSH1 expression in HUVEC-vector and HUVEC-NRP2 cells was detected by Western blot analysis (left panels). SSH1 expression in scramble siRNA- or NRP2 siRNA-treated cells was detected by Western blot analysis (right panels). C HUVEC-vector and HUVEC-NRP2 cells were treated with scramble or SSH1 siRNA. Cells were then lysed and subjected to Western blot analysis with the indicated antibodies. D The fluorescent signals of F-actin (green) and nuclei (blue) are shown (× 1000). E The migratory properties of the cells were analysed by the wound-healing assay. The data are presented as averages from 3 independent experiments (***P ≤ 0.001 by Student’s t test)