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. 2020 Sep 23;11:409. doi: 10.1186/s13287-020-01903-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Schematic representation of the stepwise differentiation of iPSC to choroidal endothelial cells. (a) Fibroblasts obtained from patient biopsies were reprogrammed to induced pluripotent stem cells (iPSCs). iPSCs were transduced with a lentiviral vector (b) expressing the endothelial cell-specific promoter CDH5 driving expression of GFP and zeocin resistance. Forty-eight hours post-transduction, cells were placed under selection with blasticidin to select for positively transduced iPSCs. Following selection, iPSC colonies were expanded prior to stepwise choroidal endothelial cell (CEC) differentiation as outlined in (c)