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CDH5-GFP-positive cells are observed at day 11 of differentiation. (a) CDH5-GFP expressing cells are observed on day 11 (a, d), day 12 (b, e), and day 13 (c, f) during stepwise differentiation accompanied by a characteristic cobblestone morphology (d–f) which closely resembles choroidal endothelial cells immortalized from a healthy human donor eye (g). Scale bars = 400 μM. Note that inlay in (c) is a high-magnification image taken from the same field
