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. 2020 Sep 23;18:131. doi: 10.1186/s12915-020-00866-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — ACBE-mediated base editing effects with different lengths of the linker peptides. a Schematic representation of ACBE, ACBE-0C, ACBE-20C, ACBE-16N, and ACBE-48 N architecture. The red square frame indicated the change of linkers. b Summary of flow cytometry results of HEK293-EGFP cells transfected ACBE variants with EGFP-Stop1 and EGFP-Stop2. c, d Summary of ACBE variants-mediated base editing patterns and efficiencies of all C and A in P53-G7 (c) and P53-G8 (d) targeting sites. e, f Summary of ACBE-mediated simbase editing patterns and efficiencies of all C and A in P53-G7 (e) and P53-G8 (f) sites using sgRNAs with different spacer lengths. The abovementioned values and error bars indicate mean ± s.e.m. of three independent experiments. The controls were untreated cell samples