Fig. 1.

DEGs and KEGG pathway analysis in the three time points of the OS. a–c The volcano plot for DEGs of osteogenic differentiation at d2, d3, and d4, respectively. The red point indicated upregulated genes and the green represented downregulation while the blue meant no difference. (DEGs were identified as differentially expressed when P.value < 0.05 and fold change (FC) ≥ 2). d–f Top 10 of DEG enrichment pathway resulted at the at d2, d3, and d4 of the OS, respectively. Point size means the gene numbers while the point color represents the P value (P value < 0.05). OS osteogenesis, DEGs differentially expressed genes, KEGG Kyoto Encyclopedia of Genes and Genomes, d day