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. 2020 Sep 22;39:196. doi: 10.1186/s13046-020-01695-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — LPP-AS2 facilitates tumor progression by regulating expression of EGFR. a Corresponding mRNAs in co-expression analysis network of LPP-AS2. Upregulated mRNAs are shown in red and downregulated mRNAs are shown in blue. b Relative expression of EGFR in 17 glioblastoma tissues and 17 normal brain tissues by RT-qPCR. c Kaplan-Meier analysis of overall survival in glioma patients with low (n = 203) and high (n = 203) level of EGFR. P value was calculated by Mantel-Cox log rank test. d The Pearson correlation between LPP-AS2 level and EGFR level was measured in the same set of glioblastoma tissues. The ΔCt values (normalized to GAPDH) were subjected to Pearson correlation analysis (R = 0.93, P < 0.0001). e Knockdown of LPP-AS2 significantly inhibited EGFR expression in U251 and SHG44 cells. f Protein level of EGFR in U251 and SHG44 cells after treated with siRNAs of LPP-AS2. g Relative expression of EGFR in U251 and SHG44 cells transfected with pEGFP-C1/EGFR alone or co-transfection with siRNAs of LPP-AS2. h CCK8 assays in U251 and SHG44 cell treated with pEGFP-C1/EGFR alone or co-transfection of si-LPP-AS2–1. i, j Histogram of migration and invasion assays in U251 and SHG44 cells treated with pEGFP-C1/EGFR alone or co-transfection of si-LPP-AS2–1. k Colony formation assay in U251 and SHG44 cells treated with pEGFP-C1/EGFR alone or co-transfection of si-LPP-AS2–1. l Histogram of cell apoptosis rate in U251 and SHG44 cells treated with pEGFP-C1/EGFR alone or co-transfection of si-LPP-AS2–1. Error bars, s.e.m. from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ^#P < 0.05; ^##P < 0.01; ^###P < 0.001; ^####P < 0.0001 (Student’s t test)