Fig. 4.

Extracellular histones reduce selectively TJ and AJ expression in the hippocampus. a Western blots of ZO-1, occludin, and VE-cadherin in cerebral cortex and hippocampus homogenates at 24 h post-saline or histone treatment. b Quantification of their protein abundance relative to ß-actin loading control. Mann–Whitney; *P < 0.05; mean ± S.E.M.; n = 4 mice per group. For gel source data, see Supplementary Fig. 4. c, d Electron microscopy micrographs showing capillaries in cerebral cortex and hippocampus from saline- and histones-treated mice at 24 h post-injection showing endothelial cells lining the capillary lumen and joined by TJs (arrowheads). Scale bar = 0.5 μm; inserts (100 nm, 200 nm)