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. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: Cell. 2020 Jul 24;182(4):992–1008.e21. doi: 10.1016/j.cell.2020.06.037

Figure 1. Self-Reporting Transposons Are Mapped More Efficiently from RNA Compared to DNA.

Figure 1.

(A) Schematics of a self-reporting piggyBac transposon with puromycin reporter gene (PB-SRT-Puro) and undirected (PBase) and SPI-directed (SP1-PBase) piggyBac transposases.

(B) Molecular workflow for mapping SRTs from bulk RNA libraries.

(C) Overlap of SRTs recovered by DNA- or RNA-based protocols in HCT-116 cells.

(D) Distribution of insertions with respect to genetic annotation between SRT libraries prepared from either DNA or RNA. TR, terminal repeat; Puro, puromycin; PAS, polyadenylation signal.